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ATP synthase is an essential enzyme responsible for the production of ATP, the primary energy currency of cells. In eukaryotic cells, ATP synthase is predominantly located in the inner mitochondrial membrane. This positioning is crucial because the enzyme utilizes the proton gradient generated by the electron transport chain during cellular respiration. As protons flow back into the mitochondrial matrix through ATP synthase, it catalyzes the conversion of ADP and inorganic phosphate into ATP.

In addition to its mitochondrial presence, ATP synthase can also be found in the thylakoid membranes of chloroplasts in plant cells, where it plays a similar role in photosynthesis. Understanding its location helps underscore the importance of organelle membranes in energy metabolism, illustrating how ATP synthase operates at these critical sites where energy conversion processes occur.

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